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Related Experiment Videos

PET imaging of transgene expression.

D C MacLaren1, T Toyokuni, S R Cherry

  • 1The Crump Institute for Biological Imaging, UCLA School of Medicine, Los Angeles, California, USA.

Biological Psychiatry
|September 9, 2000
PubMed
Summary

We developed noninvasive positron emission tomography (PET) imaging to track gene expression in living animals. This technique allows for repetitive, quantitative assessment of transgene expression, crucial for gene therapy and transgenic studies.

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Area of Science:

  • Biomedical imaging
  • Molecular biology
  • Gene therapy

Background:

  • Assaying transgene expression is critical for transgenic animal studies and gene therapy.
  • Traditional methods require invasive procedures like biopsies or subject sacrifice.
  • Noninvasive, repetitive methods are needed to monitor gene expression in vivo.

Purpose of the Study:

  • To develop noninvasive techniques for determining the location, duration, and magnitude of transgene expression in living animals.
  • To enable quantitative and repetitive assessment of gene expression for research and clinical applications.

Main Methods:

  • High-resolution positron emission tomography (PET) scanning instrumentation.
  • Development and validation of two PET reporter gene imaging systems.

Related Experiment Videos

  • In vivo imaging of gene expression in murine viral gene transfer and transgenic models.

    • Main Results:

    • Demonstrated the ability to noninvasively, quantitatively, and repetitively image gene expression in vivo.
    • Compared the utility of two distinct PET reporter gene systems.
    • Validated the PET approach for monitoring transgene expression in relevant animal models.

    Conclusions:

    • Radionuclide-based approaches, specifically PET, offer superior sensitivity and quantitation for in vivo gene expression imaging compared to optical or magnetic methods.
    • PET reporter gene imaging is a powerful tool for studying transgenic animals and evaluating gene therapies.
    • Alternative methods like radiolabeled antibodies and oligonucleotides show potential for imaging specific proteins and RNA messages directly.