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Updated: Jul 29, 2026
An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis
Published on: January 11, 2017
[A preliminary analysis on a group of low molecular weight RNases in wheat and related species]
1National Key Laboratory for Plant Cell and Chromosome Engineering, Chinese Academy of Sciences, Beijing, China.
Abstract:
By employing RNase activity gel analysis, we detected a novel group of RNases in wheat and related species. The molecular weight of the RNases varied from 8-14 kDa, which was lower than that of most plant RNases characterized previously. The small RNases expressed abundantly in seedlings and differed in their optimal pH and ionic requirement in digesting RNA substrate. Several members of the small RNases were detected in dormant and germinating wheat seeds. During germination, the activity of two RNases did not change significantly whereas that of the other two RNases showed a gradual pattern of decrease and increase, respectively.
