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Multiple-site optical recording of mouse brainstem evoked by vestibulocochlear nerve stimulation
1Department of Otolaryngology, Kansai Medical University, Fumizono-cho 10-15, Moriguchi, 570-8507, Osaka, Japan.
Abstract:
We used optical imaging to investigate the mouse cochlear and vestibular nucleus in brainstem slices using a voltage-sensitive dye, RH 155. As a result, the spatiotemporal patterns of excitatory propagation were shown. These optical signals consisted of two components consisting of a spike-like fast signal and a long-lasting slow signal. All responses were abolished by tetrodotoxin. The slow signals were eliminated under a Ca(2+)-free solution. In addition, synaptic fatigue was also observed. The present study indicated the feasibility of optical recording for visually revealing the synaptic transmission in both the vestibular and cochlear nucleus.