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One-channel Cell-attached Patch-clamp Recording
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One-channel Cell-attached Patch-clamp Recording

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Functional localization of single active ion channels on the surface of a living cell

Y E Korchev1, Y A Negulyaev, C R Edwards

  • 1Division of Medicine, Imperial College School of Medicine, MRC Clinical Sciences Centre, Hammersmith Campus, Du Cane Road, London W12 0NN, UK.

Nature Cell Biology
|September 12, 2000
PubMed

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Ion channels are specialized proteins on the plasma membrane that allow charged ions to pass down their electrochemical gradient. Their main function is to maintain the membrane potential which is critical for cell viability. These channels are either gated or non-gated and can transport more than a thousand ions within milliseconds for the cellular event to occur.
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Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
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