Related Experiment Video
Updated: Jul 6, 2026

An Affordable HIV-1 Drug Resistance Monitoring Method for Resource Limited Settings
Published on: March 30, 2014
An optimized protocol for analysis of EST sequences
1The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA.
Abstract:
The vast body of Expressed Sequence Tag (EST) data in the public databases provide an important resource for comparative and functional genomics studies and an invaluable tool for the annotation of genomic sequences. We have developed a rigorous protocol for reconstructing the sequences of transcribed genes from EST and gene sequence fragments. A key element in developing this protocol has been the evaluation of a number of sequence assembly programs to determine which most faithfully reproduce transcript sequences from EST data. The TIGR Gene Indices constructed using this protocol for human, mouse, rat and a variety of other plant and animal models have demonstrated their utility in a variety of applications and are freely available to the scientific research community.
Related Concept Videos
RNA Stability
Maxam-Gilbert Sequencing
Challenges of the Maxam-Gilbert Method
The...

