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Common enterobacterial antigen in Yersinia enterocolitica
Acta Pathologica Et Microbiologica Scandinavica. Section B, Microbiology
|August 1, 1975
Summary
The common enterobacterial antigen (CA) is present in Yersinia enterocolitica and Yersinia pseudotuberculosis. This finding is crucial for understanding bacterial antigen detection and serological testing methods.
Area of Science:
- Microbiology
- Immunology
- Bacteriology
Background:
- The common enterobacterial antigen (CA) is a shared antigen among Enterobacteriaceae.
- Accurate detection of bacterial antigens is vital for diagnostics and understanding host-pathogen interactions.
Purpose of the Study:
- To investigate the production of the common enterobacterial antigen (CA) by various bacterial strains, particularly Yersinia enterocolitica (Y.e.).
- To assess the utility of anti-CA antibodies in serological assays for Yersinia species.
Main Methods:
- Production of anti-CA antibodies in rabbits using E. coli O 14.
- Sensitization of sheep erythrocytes with CA from S. typhimurium extract.
- Detection of CA in bacterial heat extracts using absorption and haemagglutination inhibition tests.
Main Results:
- Common enterobacterial antigen (CA) was detected in heat extracts of all examined Yersinia enterocolitica strains and Yersinia pseudotuberculosis.
- CA was not found in extracts from Pasteurella multocida, Francisella tularensis, Brucella abortus, Acinetobacter calcoaceticus, or Pseudomonas aeruginosa.
- Anti-CA antibodies were successfully raised using a CA-containing Y.e. fraction, but not with whole Y.e. bacteria.
Conclusions:
- Yersinia enterocolitica and Yersinia pseudotuberculosis strains produce the common enterobacterial antigen (CA).
- The presence of CA in Yersinia species has implications for the specificity and sensitivity of serological tests, particularly indirect haemagglutination assays for detecting antibodies to Y.e. O antigens.