Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

E2F4 is essential for normal erythrocyte maturation and neonatal viability.

P O Humbert1, C Rogers, S Ganiatsas

  • 1Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA.

Molecular Cell
|September 13, 2000
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Equality, Diversity and Inclusivity dynamics and commitment profiles of comparator clinical Radiography and related medical radiation science journals.

Radiography (London, England : 1995)·2025
Same author

Establishing a Pan-European, multi-disciplinary taphonomic research Infrastructure: The 'UK-Netherlands decomposition experimental research (UNDER) Group'.

Science & justice : journal of the Forensic Science Society·2025
Same author

Comparing Hearing Loss in Children with Adults Living with Sickle Cell Disease and Sickle Cell Traits.

Nigerian journal of clinical practice·2024
Same author

Background levels of body fluids and DNA on the shaft of the penis and associated underpants in the absence of sexual activity.

Science & justice : journal of the Forensic Science Society·2023
Same author

Targeted gene expression profiling predicts meningioma outcomes and radiotherapy responses.

Research square·2023
Same author

Scoping Review of Predisposing Factors Associated with Sensorineural Hearing Loss in Sickle Cell Disease.

West African journal of medicine·2023

Loss of E2F4 in mice did not affect cell cycle but caused developmental defects, including craniofacial abnormalities and erythroid maturation issues, highlighting its role in development.

Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Genetics

Background:

  • The retinoblastoma protein (pRB) is crucial for regulating cell proliferation via E2F transcription factors.
  • E2F4 is the predominant member of the E2F family, but its specific in vivo functions remain incompletely understood.

Purpose of the Study:

  • To investigate the in vivo role of E2F4 in mouse development and cell cycle control.
  • To determine the consequences of E2F4 loss on proliferation, cell cycle arrest, and organismal development.

Main Methods:

  • Generation of a knockout mouse model lacking the E2F4 gene (E2f4-/-).
  • Phenotypic analysis of E2f4-/- mice, including assessment of cell cycle, proliferation, craniofacial development, and erythroid maturation.

Related Experiment Videos

Main Results:

  • E2F4 deficiency did not result in detectable defects in cell cycle arrest or proliferation.
  • E2f4-/- mice exhibited embryonic lethality due to increased susceptibility to infections, linked to craniofacial defects.
  • A cell-autonomous defect in late-stage erythroid maturation was observed in E2f4-/- mice, leading to various erythroid abnormalities.

Conclusions:

  • E2F4 is essential for normal mammalian development, despite not being critical for cell cycle arrest or proliferation control.
  • E2F4 plays a significant role in regulating erythrocyte development and maturation.
  • These findings reveal a novel function for E2F4 in development, mediated by the pRB tumor suppressor pathway.