Related Experiment Video
Updated: Jul 18, 2026

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy (f3D-SIM)
Published on: September 29, 2014
Myosin V orientates the mitotic spindle in yeast
H Yin1, D Pruyne, T C Huffaker
1Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA.
Abstract:
Coordination of spindle orientation with the axis of cell division is an essential process in all eukaryotes. In addition to ensuring accurate chromosomal segregation, proper spindle orientation also establishes differential cell fates and proper morphogenesis. In both animal and yeast cells, this process is dependent on cytoplasmic microtubules interacting with the cortical actin-based cytoskeleton, although the motive force was unknown. Here we show that yeast Myo2, a myosin V that translocates along polarized actin cables into the bud, orientates the spindle early in the cell cycle by binding and polarizing the microtubule-associated protein Kar9 (refs 7-9). The tail domain of Myo2 that binds Kar9 also interacts with secretory vesicles and vacuolar elements, making it a pivotal component of yeast cell polarization.
Related Concept Videos
Cytoskeletal Proteins in Bacteria
Special Staining Techniques
Flagella and Motility in Bacteria
Bacterial Phylum Verrucomicrobiota
Microbes in Food Production

