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Updated: Oct 13, 2026

Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay
Published on: August 26, 2018
Alternative splicing of insulin-like growth factor I mRNA is developmentally regulated in the rat and mouse with
1Department of Animal Science, University of California, Davis 95616, USA.
Abstract:
The synthesis of an IGF-I complementary RNA (cRNA) standard containing the primer sequences for each of the four IGF-I mRNA alternative transcript forms (class 1Ea, 1Eb, 2Ea and 2Eb) and a polyA+ tail allowed the determination of the absolute abundance of each form in growing rats and mice by quantitative RT-PCR. In rat liver, class 1Ea mRNA was the most abundant form representing 90% of the total IGF-I mRNA. Though the relative proportion was maintained, the absolute abundance was maximal at 4 weeks of age and had declined by 6 weeks. In contrast, class 2Ea mRNA was the predominant transcript in mouse liver (70% of total IGF-I mRNA) and the 2Eb mRNA form was also 10-fold higher than that detected in rat liver. These results suggest that rats and mice differ both in their transcription initiation at two leader exons and in their alternative splicing activities for exon 5.
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