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Technical note: a technique for multiple liver biopsies in neonatal calves.
K S Swanson1, N R Merchen, J W Erdman
1Division of Nutritional Sciences, University of Illinois, Urbana 61801, USA.
Journal of Animal Science
|September 14, 2000
Summary
A new, rapid liver biopsy technique was developed for neonatal calves, allowing repeated sampling for research. This safe procedure enables monitoring of liver changes over time in young cattle.
Area of Science:
- Veterinary Medicine
- Animal Science
- Surgical Techniques
Background:
- Repeated liver biopsies are crucial for monitoring animal health and research.
- Existing methods may be invasive or unsuitable for repeated use in neonatal animals.
Purpose of the Study:
- To develop and validate a rapid, safe, and repeatable liver biopsy technique for neonatal calves.
- To assess the feasibility of monitoring liver composition changes over time in individual calves.
Main Methods:
- A novel biopsy trocar was fabricated and tested in neonatal calves.
- Procedures involved sedation, local anesthesia, and a minimally invasive trocar insertion.
- Biopsies were performed on days 4, 9, 15, 21, and 28 of life.
Main Results:
- The procedure was rapid (approx. 20 min) and could be performed by a single individual.
- Minimal complications were observed in 33 calves undergoing 156 biopsies.
- Liver samples (75-150 mg wet weight) were consistently obtained.
Conclusions:
- The developed liver biopsy technique is safe, rapid, and repeatable in neonatal calves.
- This method facilitates longitudinal studies on liver composition and enzyme activity.
- It offers a valuable tool for veterinary research involving young cattle.