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Updated: Aug 13, 2026

Expression Analysis of Mammalian Linker-histone Subtypes
Published on: March 19, 2012
Resolution of allelic and non-allelic variants of histone H1 by cation-exchange-hydrophilic-interaction
C A Mizzen1, A J Alpert, L Lévesque
1Centre for Research in Neurodegenerative Diseases, University of Toronto, Ontario, Canada. cam8y@virginia.edu
Abstract:
A mixed-mode high-performance liquid chromatography (HPLC) method that resolves the six known non-allelic variants of chicken erythrocyte histone H1 is described. Common, but previously unknown, allelic variants of H1 that comigrate in polyacrylamide gel electrophoresis are also resolved. The resolution of H1 variants achieved by this method should be useful in determining the functional significance of H1 sequence heterogeneity and in analyses of post-translational modification of H1. Furthermore, the principles behind the separation should be applicable to analyses of polymorphism in other proteins.
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