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hupS and hupL constitute a transcription unit in the cyanobacterium Nostoc sp. PCC 73102
P Lindberg1, A Hansel, P Lindblad
1Department of Physiological Botany, EBC, Uppsala University, Sweden. pia.lindberg@ebc.uu.se
Abstract:
The heterocystous cyanobacterium Nostoc sp. PCC 73102 possesses an uptake hydrogenase encoded by hupS and hupL. The genes are transcribed in cells grown under N2-fixing but not under non-N2-fixing conditions. We characterised the transcription of hupS and hupL in further detail. A transcription start site was located at a position 259 bp upstream from the hupS translation start. As shown by RT-PCR, hupS and hupL constitute a single transcript in Nostoc sp. PCC 73102. The possible function of short, tandemly repeated repetitive sequences between hupS and hupL, enabling a folding of the entire intergenic region into a hairpin, is discussed.