Preliminary studies on the prolongation of the survival of mouse skin allografts cultured on a solid medium

Skin allografts were transferred between individuals of white Swiss mice. No first set reaction was observed, but a second set reaction which resulted in accelerated rejection of the graft, could be induced by a second graft transplanted 2 weeks after the first. However, the histoincompatibility of the graft which causes the rejection, could be temporarily weakened when a graft had been cultured "in vitro" for at least 6 weeks prior to grafting. Nevertheless, histocompatibility differences between two different species e.g. man and mouse, could not be weakened at all by the tissue culture method.