Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Instrumentation for flow cytometry.

G V Chapman1

  • 1BD Biosciences, 4 Research Park Drive, Macquarie University Research Park, NSW 2113, North Ryde, Australia. graeme_chapman@bd.com

Journal of Immunological Methods
|September 15, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Monitoring dendritic cells in clinical practice using a new whole blood single-platform TruCOUNT assay.

Journal of immunological methods·2004
Same author

Antibody penetration of viable human cells. I. Increased penetration of human lymphocytes by anti-RNP IgG.

Clinical and experimental immunology·1991
Same author

Flow cytometry with crystal violet to detect intracytoplasmic fluorescence in viable human lymphocytes. Demonstration of antibody entering living cells.

Journal of immunological methods·1987
Same author

Release of galactosyltransferase from human platelets and a subset of monocytes in culture.

Blood·1986
Same author

Uremic middle molecules.

The International journal of artificial organs·1981
Same author

Uremic middle molecules: separation and quantitation.

Artificial organs·1981
Same journal

Optimized intracellular flow cytometry panel enables CD4 and CD8 T cell cytokine profiling in Syrian hamsters.

Journal of immunological methods·2026
Same journal

Isosulfan blue sentinel lymph node biopsy enables reliable lymph node harvest and multicolor flow cytometry in mice.

Journal of immunological methods·2026
Same journal

Type-specific antibody detection of herpes simplex virus types 1&2 (HSV-1&2) in fingerstick blood at point-of-care sites by a rapid and sensitive lateral flow immunochromatographic assay.

Journal of immunological methods·2026
Same journal

Development of chimeric DGP-IgG antibodies as quality control for celiac disease diagnosis.

Journal of immunological methods·2026
Same journal

Stepwise single-cell-resolved deep immunophenotyping pipeline to characterise immune heterogeneity and functionality in health and disease.

Journal of immunological methods·2026
Same journal

Performance evaluation of Meso Scale Discovery (MSD) quantitative serological assays for detection of binding (IgG, IgA, IgM) and ACE2 inhibitory antibody levels for SARS-CoV-2.

Journal of immunological methods·2026
See all related articles

Advancements in technology have led to more complex yet user-friendly flow cytometers capable of analyzing multiple parameters. This review details commercially available flow cytometer analyzers and sorters with specifications from October 1999.

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Instrumentation

Background:

  • Technological progress in fluidics, optics, electronics, lasers, computing, and software has significantly advanced flow cytometry.
  • The development of novel fluorescent dyes has enhanced instrument flexibility and data acquisition capabilities.

Purpose of the Study:

  • To provide a comprehensive overview of commercially available flow cytometer instruments.
  • To detail the specifications of flow cytometer analyzers and sorters as of October 1999.

Main Methods:

  • Review of commercially available flow cytometer instruments.
  • Compilation of specifications for analyzers and sorters.

Main Results:

  • Flow cytometers have become more complex but easier to operate due to technological integration.

Related Experiment Videos

  • Instruments can now collect correlated data across eight or more parameters simultaneously.
  • A detailed description of available analyzers and sorters is presented.

    • Conclusions:

    • The evolution of flow cytometry technology has resulted in more sophisticated and accessible instruments.
    • The availability of diverse instruments caters to a wide range of analytical needs.
    • This review serves as a reference for instruments available in late 1999.