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Related Experiment Videos

Flow cytometric screening of cell-based libraries.

P S Daugherty1, B L Iverson, G Georgiou

  • 1Division of Human Biology, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N., 98109, Seattle, WA, USA. pdaugher@fhcrc.org

Journal of Immunological Methods
|September 15, 2000
PubMed
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Flow cytometry enables high-throughput screening of large microbial libraries for desired protein functions. This powerful tool can isolate rare cells with specific traits, optimizing screening parameters for efficiency.

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Microbiology

Background:

  • Flow cytometry is a high-throughput screening tool for diverse applications.
  • It enables quantitative screening of protein mutants for functions like binding and stability.
  • Rare cells (frequency < 10^-6) can be isolated from heterogeneous populations.

Purpose of the Study:

  • To analyze parameters for optimizing flow cytometry screening.
  • To enable isolation of rare target cells from large libraries.
  • To quantitatively observe and optimize the screening process.

Main Methods:

  • Utilizing flow cytometry for library screening.
  • Employing cell sorting and growth amplification for rare cell isolation.
  • Analyzing screening parameters for optimization.

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Main Results:

  • Demonstrated the capability of flow cytometry to screen large libraries.
  • Showcased the isolation of rare target cells.
  • Highlighted the importance of parameter optimization for successful screening.

Conclusions:

  • Flow cytometry is a powerful tool for screening and isolating rare cells.
  • Optimization of screening parameters is crucial for isolating cells with desired traits.
  • This technique facilitates the study and engineering of protein functions.