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Related Experiment Videos

Phagocytosis: measurement by flow cytometry.

A K Lehmann1, S Sornes, A Halstensen

  • 1Institute of Medicine, University of Bergen, N-5021, Bergen, Norway. alfred.halstensen@med.uib.no

Journal of Immunological Methods
|September 15, 2000
PubMed
Summary

Flow cytometry (FCM) rapidly measures phagocytosis and oxidative burst, aiding in evaluating phagocyte function and infection susceptibility. This review details FCM methods for assessing opsonophagocytosis and its clinical applications.

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Area of Science:

  • Immunology
  • Cell Biology
  • Medical Diagnostics

Background:

  • Phagocyte dysfunction increases infection risk.
  • Flow cytometry (FCM) enables precise single-cell analysis.
  • FCM has been used for over 20 years to assess phagocyte defects and serum opsonic activity.

Purpose of the Study:

  • To review the methodological principles of FCM for quantifying phagocytosis and oxidative burst.
  • To present assays for evaluating species-specific and antigen-specific opsonophagocytosis.
  • To discuss challenges and applications of FCM in opsonophagocytosis studies.

Main Methods:

  • Utilizing flow cytometry (FCM) for rapid, reproducible single-cell measurements.
  • Employing fluorochrome staining for simultaneous biochemical and functional analysis of phagocytosis.

Related Experiment Videos

  • Developing multiparameter assays to determine antigen-specificity of opsonophagocytic responses.

    • Main Results:

    • FCM provides quantitative measurements of phagocytosis and intracellular oxidative burst.
    • Established assays evaluate species-specific and antigen-specific opsonophagocytosis.
    • Recent advancements allow for antigen-specific opsonophagocytic response analysis.

    Conclusions:

    • FCM is a powerful tool for assessing phagocyte function and opsonophagocytosis.
    • Methodological principles and challenges in FCM-based opsonophagocytosis assays are detailed.
    • Applications of FCM in understanding infection susceptibility and immune responses are presented.