Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The p53 mutation which abrogates trans-activation while maintaining its growth-suppression activity.

S J Oh1, M Y Im

  • 1Department of Biological Sciences, College of Natural Sciences, Chonnam National University, Kwangju, Korea. sjo@chonnam.chonnam.ac.kr

Molecules and Cells
|September 15, 2000
PubMed
Summary

Mutant p53 protein can suppress cell growth independently of its transcription factor role. The 240ile mutant lost trans-activation but retained growth suppression, indicating a novel mechanism for p53 in tumor suppression.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Amifampridine to treat Lambert-Eaton myasthenic syndrome.

Drugs of today (Barcelona, Spain : 1998)·2020
Same author

Effects of particulate matter on healthy human skin: a panel study using a smartphone application measuring daily skin condition.

Journal of the European Academy of Dermatology and Venereology : JEADV·2019
Same author

Osteoporotic fractures in patients with systemic lupus erythematosus and end stage renal disease.

Lupus·2017
Same author

API5 confers cancer stem cell-like properties through the FGF2-NANOG axis.

Oncogenesis·2017
Same author

Engineering the surface chemistry of lead chalcogenide nanocrystal solids to enhance carrier mobility and lifetime in optoelectronic devices.

Chemical communications (Cambridge, England)·2016
Same author

Who may benefit from robotic gastrectomy?: A subgroup analysis of multicenter prospective comparative study data on robotic versus laparoscopic gastrectomy.

European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·2016

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Cell Biology

Background:

  • The p53 protein is a crucial tumor suppressor that typically functions as a transcription factor.
  • Previous studies suggest some p53 mutants can inhibit cell growth independent of transcriptional activity.
  • Understanding the distinct functions of p53 is vital for cancer therapy development.

Purpose of the Study:

  • To investigate the relationship between the trans-activation and growth-suppressive functions of p53.
  • To analyze the specific roles of five distinct p53 mutants in cellular processes.
  • To elucidate the mechanisms underlying p53-mediated tumor suppression.

Main Methods:

  • Utilized CAT reporter assays to measure trans-activational activity of p53 mutants.

Related Experiment Videos

  • Employed colony formation assays to assess the growth-suppressive capabilities of mutants.
  • Generated isogenic cell lines expressing mutant p53 to analyze growth rates.

    • Main Results:

    • The p53 mutant 250ala retained trans-activational activity, while mutants 199stop, 240ile, 285lys, and 291asn lost this function.
    • Mutants 240ile and 250ala demonstrated retained growth suppression, unlike mutants 199stop, 285lys, and 291asn.
    • Cell lines expressing 240ile and 250ala exhibited slower growth rates compared to those with non-functional mutants.

    Conclusions:

    • Mutant p53 240ile lost its trans-activational activity but maintained growth-suppressive properties.
    • This finding highlights a potential non-transcriptional role for p53 in inhibiting tumor growth.
    • Further research is needed to fully understand the growth-suppressive mechanisms of p53 mutant 240ile.