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Related Experiment Videos

Induced sputum cellularity. Reference values and distribution in normal volunteers.

A Spanevello1, M Confalonieri, F Sulotto

  • 1Fondazione Salvatore Maugeri, Care and Research Institute, Tradate, Italy.

American Journal of Respiratory and Critical Care Medicine
|September 16, 2000
PubMed
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Induced sputum analysis in healthy individuals reveals normal reference values for airway inflammation. Macrophages and neutrophils are abundant, while eosinophils and lymphocytes are scarce, providing a baseline for disease comparison.

Area of Science:

  • Pulmonary Medicine
  • Respiratory Cell Biology
  • Clinical Immunology

Background:

  • Sputum induction is a noninvasive method for assessing airway inflammation.
  • Defining reference values for induced sputum cell counts in healthy populations is crucial for accurate diagnosis.
  • Previous studies lacked comprehensive data on cell distribution in healthy subjects.

Purpose of the Study:

  • To establish reference values for induced sputum cell counts in a healthy, non-hyperresponsive, non-smoking population.
  • To characterize the distribution of various cell types in induced sputum from healthy volunteers.
  • To provide a baseline for comparing sputum cell profiles in patients with airway inflammatory diseases.

Main Methods:

  • Recruitment of 114 healthy, nonatopic, nonsmoking volunteers without airway hyperreactivity.

Related Experiment Videos

  • Sputum induction procedure to obtain airway samples.
  • Differential cell count analysis of induced sputum samples.

    • Main Results:

    • Ninety-six (84%) subjects provided adequate sputum samples for analysis.
    • Induced sputum primarily consisted of macrophages (69.2% ± 13%) and neutrophils (27.3% ± 13%).
    • Eosinophils (0.6% ± 0.8%), lymphocytes (1.0% ± 1.2%), and epithelial cells (1.5% ± 1.8%) were found in low proportions.
    • Macrophages and neutrophils exhibited normal distribution patterns, unlike other cell types.

    Conclusions:

    • Established reference ranges for induced sputum cell differential counts in healthy adults.
    • These findings provide essential normative data for diagnosing and monitoring airway inflammation.
    • The data facilitate the comparison of sputum cell profiles between healthy individuals and those with respiratory conditions.