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Transgenic methods for increasing Drosophila life span
1Department of Biological Sciences, SHS172, University of Southern California, Los Angeles, CA 90089-1340, USA. jtower@usc.edu
Mechanisms of Ageing and Development
|September 16, 2000
Abstract:
At least five different transgenic approaches have been applied to the study of Drosophila aging. There are two single component systems: transgenes with native (normal) promoters and transgenes with heterologous promoters; as well as three binary systems: 'GAL4/UAS', 'FLP-out' and 'tet-on'. These approaches vary in ability to meet several technical challenges, and the relative advantages and disadvantages of each are discussed. Using these techniques, over-expression of the hsp70, Cu/ZnSOD and MnSOD genes has each been demonstrated to increase Drosophila life span.