Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The isoelectric focusing problem analytic solution.

L L Frumin1, G V Zilberstein, S E Peltek

  • 1Proteologics (Israel) Ltd, 4 Pekeris Street, 76702, Rehovot, Israel. frumin@phys.nsu.ru

Journal of Biochemical and Biophysical Methods
|September 16, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cellulases: key properties, natural sources, and industrial applications.

Vavilovskii zhurnal genetiki i selektsii·2026
Same author

The mitochondrial genome of Dendrobaena tellermanica Perel, 1966 (Annelida: Lumbricidae) and its phylogenetic position.

Vavilovskii zhurnal genetiki i selektsii·2023
Same author

De novo assembly and analysis of the transcriptome of the Siberian wood frog Rana amurensis.

Vavilovskii zhurnal genetiki i selektsii·2022
Same author

Creation of a recombinant Komagataella phaffii strain, a producer of proteinase K from Tritirachium album.

Vavilovskii zhurnal genetiki i selektsii·2022
Same author

Production of subtilisin proteases in bacteria and yeast.

Vavilovskii zhurnal genetiki i selektsii·2021
Same author

Variation in nuclear genome size within the Eisenia nordenskioldi complex (Lumbricidae, Annelida).

Vavilovskii zhurnal genetiki i selektsii·2021
Same journal

Proceedings of the 12th International Symposium Of Chemistry, Miercurea Ciuc (Csíkszereda), Romania, October 5-8, 2006; and proceedings of the 7th International Symposium and Summer School on Bioanalysis, Pécs, Hungary, June 10-17, 2007.

Journal of biochemical and biophysical methods·2008
Same journal

12th International Symposium Of Chemistry, Miercurea Ciuc (Csíkszereda), Romania, 5th to 8th of October, 2006. 7th International Symposium and Summer School on Bioanalysis, Pécs, Hungary, 10th to 17th of June, 2007.

Journal of biochemical and biophysical methods·2008
Same journal

Optimized HPLC method for tramadol and O-desmethyl tramadol determination in human plasma.

Journal of biochemical and biophysical methods·2008
Same journal

The alternating current polarographic behavior and determination of lansoprazole and omeprazole in dosage forms and biological fluids.

Journal of biochemical and biophysical methods·2008
Same journal

Continuous-flow complete-mixing system for assessing the effects of environmental factors on colony-level coral metabolism.

Journal of biochemical and biophysical methods·2008
Same journal

Utilization of fluorescence tracer in hyperinsulinemic-euglycemic clamp test in mice.

Journal of biochemical and biophysical methods·2008
See all related articles

Isoelectric focusing (IEF) analytical solutions precisely address the focusing problem. These solutions reveal how sample fractions move and narrow within electric fields, aiding electrophoretic experiment calculations.

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Physical Chemistry

Background:

  • Isoelectric focusing (IEF) is a powerful protein separation technique.
  • The focusing problem, where sample zones broaden, has historically limited IEF resolution.
  • Precise analytical solutions are needed to understand and optimize IEF dynamics.

Purpose of the Study:

  • To analytically solve the focusing problem in isoelectric focusing.
  • To determine the precise laws governing fraction movement and narrowing.
  • To enhance the development of calculation methods for electrophoretic experiments.

Main Methods:

  • Analytical mathematical modeling of the focusing process.
  • Derivation of precise solutions for zone dynamics in an electric field gradient.

Related Experiment Videos

  • Application of derived solutions to electrophoretic experimental design.

    • Main Results:

    • An exact analytical solution to the IEF focusing problem was obtained.
    • The laws governing the movement and narrowing of sample fractions were precisely determined.
    • The developed solutions provide a theoretical basis for improving electrophoretic calculations.

    Conclusions:

    • The analytical solutions presented effectively resolve the IEF focusing problem.
    • Understanding fraction dynamics in electric field gradients is crucial for optimizing separation.
    • This work advances the computational methods applicable to electrophoretic experiments.