Regulation of expression of branched-chain alpha-keto acid dehydrogenase subunits in permanent cell lines
1Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA.
Methods in Enzymology
|September 16, 2000
Abstract:
The rat hepatoma cell line H4IIEC3 has demonstrated a response to both insulin and glucocorticoids in its accumulation of BCKAD subunit RNAs. It is amenable to BCKAD promoter minigene transfection analyses, demonstrating positive (glucocorticoids) and negative (insulin) regulatory effects. These cells can therefore be used as a model to identify cis-acting sites responsible for regulation of BCKAD subunit promoter activity.
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