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Updated: Oct 13, 2026
A Protocol for Analyzing Hepatitis C Virus Replication
Published on: June 26, 2014
A highly sensitive and specific enzyme-linked immunosorbent assay of antibodies to hepatitis C virus
1Department of Molecular Biology and Genetics, Science Faculty, Bilkent University, Ankara, Turkey.
Abstract:
In this study, a 178 amino acids long portion of the hepatitis C virus (HCV) core gene was cloned, sequenced, expressed in Escherichia coli, and purified. The resulting antigen (C178) was tested with human sera enzyme-linked immunosorbent assay (ELISA) in order to assess its ability to diagnose HCV. It was shown by ELISA that 92% of the patients sera, diagnosed previously by a 3rd generation enzyme immunoassay (EIA) as HCV-positive, had antibodies against the C178 antigen. This antigen gave no false positive results when tested with anti-HCV-negative sera.
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