Related Experiment Video
Updated: Oct 13, 2026

Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR
Published on: July 22, 2012
Detection of small round structured viruses in stool specimens from outbreaks of gastroenteritis by electron
1Faculty of Health Science, Kyushu University of Health and Welfare, Miyazaki, Japan. rootsu@phoenix.ac.jp
Abstract:
In testing 60 stool specimens small round structured virus (SRSV) particles were detected in 35 (58%) specimens from all 17 outbreaks of gastroenteritis by electron microscopy (EM), while SRSV genes were found in 36 (60%) specimens from 15 outbreaks by reverse transcription-polymerase chain reaction (RT-PCR) by use of 2 primer pairs. Specimens from 2 outbreaks were found SRSV particles-positive by EM but SRSV genes-negative by RT-PCR. EM remains the basic examination method for diagnosis of SRSV agents.
Related Concept Videos
Real Time RT-PCR
The real-time quantification of the number of amplified products is...
Bacterial Gastroenteritis

