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Detection of the high-level aminoglycoside resistance gene aph(2")-Ib in Enterococcus faecium
1Research and Medical Service, John D. Dingell VA Medical Center, and Wayne State University School of Medicine, Detroit, Michigan 48201, USA.
Antimicrobial Agents and Chemotherapy
|September 19, 2000
Abstract:
A new high-level gentamicin resistance gene, designated aph(2")-Ib, was cloned from Enterococcus faecium SF11770. The deduced amino acid sequence of the 897-bp open reading frame of aph(2")-Ib shares homology with the aminoglycoside-modifying enzymes AAC(6')-APH(2"), APH(2")-Ic, and APH(2")-Id. The observed phosphotransferase activity is designated APH(2")-Ib.