Related Experiment Videos
Methionine interference in rapamycin production involves repression of demethylrapamycin methyltransferase and
1Fermentation Microbiology Laboratory, Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Antimicrobial Agents and Chemotherapy
|September 19, 2000
Abstract:
In a chemically defined medium, L-methionine decreased production of rapamycin and increased that of demethylrapamycin. Growth with L-methionine yielded cells with a lower ability to convert demethylrapamycin to rapamycin and decreased the level of S-adenosylmethionine synthetase and S-adenosylmethionine. Thus, methionine represses at least one methyltransferase of rapamycin biosynthesis and S-adenosylmethionine synthetase.