Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Highly efficient gene transfer into preterm CD34 hematopoietic progenitor cells.

L E Shields1, H P Kiem, R G Andrews

  • 1Division of Perinatal Medicine, Department of Obstetrics and Gynecology, University of Washington School of School of Medicine, Seattle, WA 98195-6460, USA.

American Journal of Obstetrics and Gynecology
|September 19, 2000
PubMed
Summary

Gene transfer into early fetal umbilical cord blood CD34(+) cells is as efficient as in term cells. This suggests potential for in utero gene therapy, though further in vivo studies are needed.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hit-and-run programming of therapeutic cytoreagents using mRNA nanocarriers.

Nature communications·2017
Same author

Cyclophosphamide-based in vivo T-cell depletion for HLA-haploidentical transplantation in Fanconi anemia.

Pediatric hematology and oncology·2012
Same author

Myelodysplasia in 2 pig-tailed macaques (Macaca nemestrina) associated with retroviral vector-mediated insertional mutagenesis and overexpression of HOXB4.

Veterinary pathology·2010
Same author

Myeloablative irradiation in non-human primates.

Journal of medical primatology·2009
Same author

Selective reduction of multifetal pregnancy using high-intensity focused ultrasound in the rabbit model.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2005
Same author

Chimerism analysis by sex determining region Y (SRY) and major histocompatibility complex markers in non-human primates using quantitative real-time polymerase chain reaction.

Tissue antigens·2005

Area of Science:

  • * Hematology
  • * Gene Therapy
  • * Developmental Biology

Background:

  • * Retrovirus-mediated gene transfer is effective for transducing CD34(+) cells from term umbilical cord blood.
  • * Hematopoietic progenitor cells (HPCs) are crucial for blood formation and are a target for gene therapy.
  • * Umbilical cord blood (UCB) is a rich source of HPCs, with varying characteristics depending on gestational age.

Purpose of the Study:

  • * To compare the efficiency of retrovirus-mediated gene transfer into CD34(+) HPCs from early-gestation (23-28 weeks) and term-gestation (37-41 weeks) UCB.
  • * To evaluate the potential of early fetal UCB as a source for gene therapy.

Main Methods:

  • * CD34(+) cells were isolated from cryopreserved early and term UCB using fluorescence-activated cell sorting.

Related Experiment Videos

  • * Cells were transduced using a gibbon ape leukemia virus pseudotype vector (LAPSN [PG13]) in the presence of growth factors and fibronectin fragment CH-296.
  • * Transduction efficiency was assessed by histochemical staining for alkaline phosphatase activity in colony-forming cells.

    • Main Results:

    • * Gene transfer efficiencies were comparable between early (58.4% +/- 11.8%) and term (63.2% +/- 12.5%) gestation CD34(+) HPCs.
    • * Alkaline phosphatase staining indicated similar transduction rates in granulocyte-macrophage colony-forming units and erythroid burst-forming units.

    Conclusions:

    • * CD34(+) HPCs from midgestation fetal blood can be transduced with high efficiency using established methods.
    • * The early fetus may be a favorable target for gene therapy due to a higher number of progenitor cells and greater proliferative capacity.
    • * Further in vivo studies in animal models are necessary to explore clinically applicable in utero gene therapy approaches.