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Related Experiment Videos

Hyperfast, early cell response to ionizing radiation.

V Ponette1, C Le Péchoux, E Deniaud-Alexandre

  • 1Unité 350 INSERM, Institut Curie-Biologie, Orsay, France.

International Journal of Radiation Biology
|September 19, 2000
PubMed
Summary

Rapid cellular responses to radiation, including DNA repair and chromatin remodeling, influence radio-sensitivity and cell death, but do not directly correlate with apoptosis or DNA strand breaks.

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Area of Science:

  • Radiobiology
  • Cellular Radiation Response

Background:

  • Radio-sensitivity can oscillate rapidly after irradiation.
  • The mechanisms underlying these oscillations are not fully understood.

Purpose of the Study:

  • To investigate the correlation between early radio-sensitivity oscillations and cellular damage markers.
  • To explore the role of apoptosis, DNA strand breaks, and lipid signaling in these oscillations.

Main Methods:

  • Human tumor cells and Chinese hamster fibroblasts were used.
  • Split-dose, high dose-rate electron pulse irradiation was applied.
  • Clonogenic assays, DNA cleavage analysis, and microscopy were employed.

Main Results:

  • Oscillatory radio-sensitivity did not correlate with increased DNA breaks or apoptosis.

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  • N-acetylcysteine and lipid signaling inhibitors did not affect the oscillatory response.
  • The response correlated with phenotypic alterations leading to mitotic or delayed cell death.

    • Conclusions:

    • Early DNA damage recognition and repair may induce rapid chromatin remodeling.
    • This remodeling could enhance chromosome damage upon re-irradiation.
    • High dose-rate irradiation shows less instability than protracted irradiation.