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[Development of a quantitative PCR variant, and use of it for assessing gene expression]
Iu S Aliapkina1, Iu M Romanova, N V Alekseeva
1Gamaleya Scientific Research Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences, Moscow, Russia.
Genetika
|September 20, 2000
Abstract:
The data on the development of a quantitative PCR technique are presented, including the synthesis of modified primers, the choice of conditions for the removal of reaction-mixture components from the amplified DNA, and the colorimetric detection of the PCR product. The application of this technique to the assessment of the level of gene expression in nonculturable bacterial forms characterized by a drastically reduced metabolism is described.