Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A fully integrated monolithic microchip electrospray device for mass spectrometry

Schultz1, Corso, Prosser

  • 1Advanced BioAnalytical Services, Inc., Ithaca, New York 14850, USA. gschultz@abs-lcms.com

Analytical Chemistry
|September 20, 2000
PubMed
Summary

A novel microfabricated nozzle enables reproducible nano-electrospray from microfluidic devices for mass spectrometry. This robust silicon device offers enhanced sensitivity and stability compared to traditional capillaries.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Efficacy of Ruxolitinib in Patients With Chronic Neutrophilic Leukemia and Atypical Chronic Myeloid Leukemia.

Journal of clinical oncology : official journal of the American Society of Clinical Oncology·2019
Same author

Study of the Second Order Transitions and Acid-Base Properties of Polymers Adsorbed on Oxides by Using Inverse Gas Chromatography at Infinite Dilution.

Journal of colloid and interface science·2000
Same author

Study of the Second Order Transitions and Acid-Base Properties of Polymers Adsorbed on Oxides by Using Inverse Gas Chromatography at Infinite Dilution.

Journal of colloid and interface science·2000
Same author

Topological organic chemistry. 13. Transformation of graph adjacency matrixes to distance matrixes

Journal of chemical information and computer sciences·2000
Same author

Time- and frequency-resolved photoionisation of the allyl radical

Faraday discussions·2000
Same author

Stable and Selective Hybridization of Oligonucleotides with Unnatural Hydrophobic Bases This work was supported by the National Institutes of Health (GM 60005 to F.E.R.) and the Skaggs Institute for Chemical Biology (F.E.R. and P.G.S.). A.K.O. thanks the National Institutes of Health for a postdoctoral fellowship (F32 GM19833-01).

Angewandte Chemie (International ed. in English)·2000

Area of Science:

  • Analytical Chemistry
  • Microfluidics
  • Mass Spectrometry

Background:

  • Electrospray ionization is crucial for interfacing liquid samples with mass spectrometry.
  • Current methods using pulled capillaries can suffer from reproducibility and stability issues.
  • Microfluidic devices offer miniaturization and precise fluid control.

Purpose of the Study:

  • To develop and characterize a novel microfabricated nozzle for electrospray from microfluidic devices.
  • To evaluate the performance of the microfabricated nozzle in terms of reproducibility, stability, and sensitivity.
  • To compare the microfabricated nozzle with traditional pulled capillaries for mass spectrometry applications.

Main Methods:

  • Fabrication of micro nozzles using deep reactive ion etching on a monolithic silicon substrate.

Related Experiment Videos

  • Integration of microfluidic channels and reservoirs within the silicon device.
  • Interfacing the electrospray device with an atmospheric pressure ionization time-of-flight mass spectrometer.
  • Testing with various solvent compositions and analyte concentrations (e.g., cytochrome c).

    • Main Results:

    • Demonstrated nozzle diameters as small as 15 micrometers.
    • Achieved nozzle-to-nozzle signal reproducibility of 12% and single-nozzle stability <4% relative standard deviation (RSD).
    • Showed 1.5-3 times increased sensitivity and improved signal stability (2-4% RSD) compared to pulled capillaries.
    • Reported a signal-to-noise ratio of 450:1 for 10 nM cytochrome c in water.
    • Device lifetime exceeded 8 hours with a total volume <25 pL.

    Conclusions:

    • The microfabricated electrospray nozzle provides a reproducible, controllable, and robust platform for nano-electrospray.
    • This technology offers significant advantages in sensitivity and stability over conventional pulled capillaries for mass spectrometry.
    • The device is well-suited for integration with microfluidic separation systems, enabling miniaturized analytical workflows.