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Updated: Aug 15, 2026
A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
Published on: April 11, 2014
An improved, rapid in vitro method to measure antioxidant activity. Application On selected flavonoids and apple
A A van der Sluis1, M Dekker, R Verkerk
1Integrated Food Technology Group, Department of Agrotechnology and Food Sciences, Wageningen University, P.O. Box 8129, 6700 EV Wageningen, The Netherlands.
Abstract:
A rapid in vitro method for measuring antioxidant activity is presented, which enables the evaluation of health claims and the optimization of product development with respect to health protecting compounds. Antioxidant activity is assessed in a system in which lipid peroxidation is induced in male rat liver microsomes by ascorbic acid and FeSO(4). This method has been significantly improved by enabling the use of microtiter plates and an ELISA reader. Large numbers of samples can be analyzed with good reproducibility, which is necessary when dealing with microsomes possessing biological variability. An objective mathematical procedure has been developed to translate data obtained from the lipid peroxidation assay into a value describing the antioxidant activity. As an illustration the method has been applied to measure antioxidant activity of individual flavonoids and apple juice.
