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A 2-Mb YAC/BAC-based physical map of the ovum mutant (Om) locus region on mouse chromosome 11

M Cohen-Tannoudji1, S Vandormael-Pournin, S Le Bras

  • 1Unité de Biologie du Développement, CNRS URA 1960, Institut Pasteur, 25 rue du Dr. Roux, Paris Cedex 15, 75724, France. m-cohen@pasteur.fr

Genomics
|September 21, 2000
PubMed

Insights

Researchers mapped the Om locus, the cause of DDK syndrome, to a 1400 kb region on chromosome 11. This physical map aids in identifying candidate genes for this embryonic lethal phenotype.

Area of Science:

  • Developmental Biology
  • Genetics
  • Reproductive Biology

Background:

  • DDK syndrome is an embryonic lethal phenotype caused by interactions between egg cytoplasm and paternal pronucleus.
  • The Om locus, responsible for DDK syndrome, was previously mapped to chromosome 11.

Purpose of the Study:

  • To generate a high-resolution physical map of the Om locus region on chromosome 11.
  • To precisely map genes and genetic markers within this interval.
  • To identify candidate genes for DDK syndrome.

Main Methods:

  • Construction of a physical map using yeast and bacterial artificial chromosome clones.
  • Integration of genetic and physical mapping data.
  • Mapping of known genes and expressed sequence tags (ESTs) within the defined interval.

Main Results:

  • A physical contig of approximately 2 Mb was assembled, encompassing the Om locus.
  • The maximum physical interval for the Om locus was refined to 1400 kb.
  • Eleven members of the Scya gene family were localized to the borders of the Om region.
  • Rad51l3, Schlafen 2, and EST D11Wsu78e were mapped within the Om region.

Conclusions:

  • The generated integrated map provides a detailed framework for the Om locus region.
  • This map facilitates the identification and characterization of candidate genes underlying DDK syndrome.
  • Further investigation of mapped genes may elucidate the genetic basis of this embryonic lethal phenotype.

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