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Related Experiment Videos

Multiple peptides converge to activate the same voltage-dependent current in a central pattern-generating circuit.

A M Swensen1, E Marder

  • 1Volen Center and Biology Department, Brandeis University, Waltham, Massachusetts 02454, USA.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|September 21, 2000
PubMed
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Six substances activate the same inward current in crab neurons, demonstrating convergence despite distinct receptors. Differential responses depend on receptor distribution, influencing network activity.

Area of Science:

  • Neuroscience
  • Computational Neuroscience
  • Marine Biology

Background:

  • The stomatogastric ganglion in crustaceans like Cancer borealis is a model system for studying neural circuit function.
  • Numerous neuropeptides and other substances modulate stomatogastric ganglion activity, influencing neuronal firing patterns.
  • Proctolin, a neuropeptide, induces a specific inward current with outward rectification in these neurons.

Purpose of the Study:

  • To investigate the ionic currents underlying the effects of proctolin and five other modulators on Cancer borealis stomatogastric ganglion neurons.
  • To determine if these diverse modulators converge onto a common ionic current.
  • To explore the role of receptor distribution in mediating differential neuronal responses.

Main Methods:

Related Experiment Videos

  • Voltage clamp electrophysiology was employed on stomatogastric ganglion neurons, both in intact ganglia and dissociated cell cultures.
  • Currents evoked by proctolin, C. borealis tachykinin-related peptide Ia (CabTRP Ia), crustacean cardioactive peptide, red pigment-concentrating hormone, TNRNFLRFamide, and pilocarpine were analyzed.
  • Experiments included varying extracellular calcium concentrations and using a calmodulin inhibitor to probe current properties and signaling pathways.

    • Main Results:

    • All six tested substances induced currents with similar voltage-dependent properties, characterized by outward rectification that linearized in low extracellular calcium.
    • The lateral pyloric neuron responded to all six modulators, while the ventricular dilator neuron showed a more restricted response profile.
    • Proctolin and CabTRP Ia responses occluded each other, and a calmodulin inhibitor affected responses to both, suggesting convergence onto a shared current.

    Conclusions:

    • All six modulatory substances converge on the same voltage-dependent inward current in stomatogastric ganglion neurons.
    • Despite activating different receptors, the ultimate downstream effect is mediated by this common current.
    • Differential network responses to these modulators are likely determined by the specific distribution of their respective receptors across different neurons.