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Published on: July 20, 2014
PTEN/MMAC1 expression in esophageal squamous cell carcinomas
1Department of Surgery and Surgical Basic Science, Graduate School of Medicine, Kyoto University, Sakyoku, Kyoto 606-8507, Japan.
International Journal of Oncology
|September 21, 2000
Summary
The PTEN/MMAC1 gene, a tumor suppressor, showed normal expression in esophageal cancer cell lines and tissues. This suggests PTEN/MMAC1 is rarely involved in esophageal squamous cell carcinoma development.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- The PTEN/MMAC1 gene is a tumor suppressor gene candidate.
- Mutations in PTEN/MMAC1 are found in various tumors.
- Alternative inactivation mechanisms like promoter methylation exist.
Purpose of the Study:
- To investigate abnormalities in PTEN/MMAC1 gene expression in esophageal cancer.
- To determine the role of PTEN/MMAC1 in esophageal squamous cell carcinoma.
Main Methods:
- Reverse transcription polymerase chain reaction (RT-PCR) for mRNA detection.
- Western blot analysis for protein expression.
- Immunohistochemical staining for protein localization and expression in tissues.
Main Results:
- PTEN/MMAC1 mRNA was expressed in all esophageal cancer cell lines and normal epithelial cells.
- Western blot confirmed PTEN/MMAC1 protein expression without significant differences.
- Immunohistochemistry revealed strong cytoplasmic PTEN/MMAC1 staining in both cancer and normal esophageal tissues.
Conclusions:
- PTEN/MMAC1 gene expression is not significantly altered in esophageal squamous cell carcinoma.
- PTEN/MMAC1 is rarely associated with the carcinogenesis of esophageal squamous cell carcinoma.
