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Techniques for Imaging Ca2+ Signaling in Human Sperm
07:38

Techniques for Imaging Ca2+ Signaling in Human Sperm

Published on: June 17, 2010

Confocal microscopy of intracellular calcium dynamics during fertilization

S A Stricker1

  • 1Department of Biology, University of New Mexico, Albuquerque 87131, USA. sstr@unm.edu

Biotechniques
|September 21, 2000
PubMed
Abstract

No abstract available in PubMed .

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During fertilization, an egg and sperm cell fuse to create a new diploid structure. In humans, the process occurs once the egg has been released from the ovary, and travels into the fallopian tubes. The process requires several key steps: 1) sperm present in the genital tract must locate the egg; 2) once there, sperm need to release enzymes to help them burrow through the protective zona pellucida of the egg; and 3) the membranes of a single sperm cell and egg must fuse, with the sperm...