Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Insect chitin synthase cDNA sequence, gene organization and expression.

R L Tellam1, T Vuocolo, S E Johnson

  • 1Molecular Animal Genetics Centre, CSIRO Tropical Agriculture, University of Queensland, Brisbane, Australia.

European Journal of Biochemistry
|September 21, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Horses with previous episodes of laminitis have altered insulin responses to seasonal oral sugar testing and grazing compared to horses with no known history of laminitis.

Journal of equine veterinary science·2024
Same author

Characterising the transcriptomic response of bovine peripheral blood mononuclear cells to a mycobacterial cell wall fraction.

Molecular immunology·2024
Same author

Misdiagnosed plicae palmatae.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2023
Same author

Effects of nutrient restriction on the metabolic profile of Bos indicus-influenced and B. taurus suckled beef cows.

Animal : an international journal of animal bioscience·2021
Same author

Human IL6 stimulates bovine satellite cell proliferation through a Signal transducer and activator of transcription 3 (STAT3)-dependent mechanism.

Domestic animal endocrinology·2017
Same author

Comparative analyses of whole genome sequences of Leishmania infantum isolates from humans and dogs in northeastern Brazil.

International journal for parasitology·2017

Chitin is essential for insect survival, as demonstrated by larval mortality when exposed to nikkomycin Z. Researchers identified and characterized the first arthropod chitin synthase-like protein, LcCS-1, crucial for chitin production in insects.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Entomology

Background:

  • Chitin is a vital structural component in arthropod cuticles.
  • The precise mechanisms of chitin synthesis remain incompletely understood.
  • Nikkomycin Z, a chitin synthase inhibitor, causes mortality in Lucilia cuprina larvae.

Purpose of the Study:

  • To investigate the essentiality of chitin in Lucilia cuprina.
  • To identify and characterize the chitin synthase-like protein (LcCS-1) in Lucilia cuprina.
  • To elucidate the structure, localization, and evolutionary conservation of LcCS-1.

Main Methods:

  • Larval mortality assays using nikkomycin Z.
  • cDNA sequencing and amino acid analysis of LcCS-1.
  • Bioinformatic analysis and mRNA localization studies.

Related Experiment Videos

Main Results:

  • Nikkomycin Z exposure resulted in concentration-dependent mortality, confirming chitin's essential role.
  • The complete cDNA and amino acid sequence of LcCS-1 were determined, revealing it as an integral membrane protein with similarities to yeast chitin synthases.
  • LcCS-1 mRNA expression was observed in all life stages, localized to the integument and trachea in larvae.
  • Homologous sequences were found in various arthropod orders and Caenorhabditis elegans, indicating evolutionary importance.

Conclusions:

  • Chitin synthesis is essential for the survival of Lucilia cuprina.
  • LcCS-1 represents the first identified arthropod chitin synthase-like enzyme, crucial for chitin production.
  • The study provides insights into the structure, function, and evolutionary conservation of chitin synthases in chitin-producing organisms.