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A method to estimate acetylcholinesterase-active sites and turnover in insects
A Charpentier1, P Menozzi, V Marcel
1Laboratoire de Synthèse et Physicochimie des Molécules d' Intérêt Biologiques, Université Paul Sabatier, Toulouse, 31062, France.
Analytical Biochemistry
|September 22, 2000
Abstract:
Acetylcholinesterase is the primary target of organophosphorous and carbamate insecticides. Quantitative changes in acetylcholinesterase are suspected to confer resistance to these insecticides, but a method to estimate the amount in insect is not available. A method using irreversible inhibitors has been developed. Among the irreversible inhibitors tested, 7-(methylethoxyphosphinyloxy)-1-methylquinolinium iodide, chlorpyrifos-ethyl-oxon, and coumaphos-oxon were found to be sufficiently potent and specific.