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Related Experiment Videos

Nociceptin effects in the airways.

C Peiser1, B J Undem, A Fischer

  • 1Humboldt University, Charité, Virchow Clinic, Department of Pediatric Pneumology and Immunology, Berlin, Germany. christian.peiser@charite.de

Peptides
|September 22, 2000
PubMed
Summary
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Nociceptin (NC), an opioid-like peptide, inhibits airway neurotransmission and smooth muscle contraction. These effects, mediated by NC receptors, are distinct from classic opioid receptors and relevant to airway physiology.

Area of Science:

  • Pharmacology
  • Neuroscience
  • Respiratory Physiology

Background:

  • Nociceptin (NC) is an opioid-like heptadecapeptide.
  • Airway neurotransmission involves acetylcholine (ACh), substance P (SP), and calcitonin gene-related peptide (CGRP).
  • Nonadrenergic-noncholinergic (NANC) bronchoconstriction is a key airway reflex.

Purpose of the Study:

  • To investigate the effects of nociceptin (NC) on airway neurotransmission and smooth muscle contraction.
  • To determine the receptor subtype involved in NC's actions in the airways.
  • To assess the physiological relevance of NC in airway function.

Main Methods:

  • Experiments conducted on isolated guinea pig and rat tracheae and bronchi.
  • Electric field stimulation (EFS) used to induce neurotransmitter release and bronchoconstriction.

Related Experiment Videos

  • Concentration-response studies with NC, NC receptor antagonist ([F/G]NC(1-13)NH(2)), and NC analogs (NCNH(2), NC(1-13)NH(2)).
  • Naloxone-insensitivity was assessed.

    • Main Results:

    • NC inhibited EFS-induced release of ACh, SP, and CGRP in a concentration-dependent manner.
    • NC inhibited EFS-induced tachykinergic NANC bronchoconstriction concentration-dependently.
    • NC-mediated effects were naloxone-insensitive but blocked by the NC receptor antagonist.
    • NC analogs mimicked the observed effects.

    Conclusions:

    • Nociceptin (NC) inhibits airway neurotransmission and smooth muscle contraction via a naloxone-insensitive mechanism.
    • These effects are mediated by a receptor distinct from classic opioid receptors, likely the NC (ORL-1) receptor.
    • NC plays a significant role in airway physiology, evidenced by its presence in airway nerves and receptor transcripts in relevant ganglia.