Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nociceptin and the micturition reflex.

A Lecci1, S Giuliani, S Meini

  • 1Pharmacology Department, Menarini Ricerche, via Sette Santi 3, 50131, Florence, Italy.

Peptides
|September 22, 2000
PubMed
Summary

Nociceptin administration inhibits the micturition reflex via capsaicin-sensitive nerves. It also shows peripheral excitatory effects on bladder contractions, indicating complex roles for nociceptin and ORL(1) receptors in micturition control.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intrabodies for protein interference in Alzheimer’s disease.

Journal of biological regulators and homeostatic agents·2014
Same author

ProNGF\NGF imbalance triggers learning and memory deficits, neurodegeneration and spontaneous epileptic-like discharges in transgenic mice.

Cell death and differentiation·2013
Same author

Effect of intrathecal administration of ET-1, ET-3 and ET(16-21) on blood pressure and micturition reflex in anesthetized rats.

Neurochemistry international·2010
Same author

Tachykinin receptors and gastrointestinal motility: focus on humans.

European review for medical and pharmacological sciences·2008
Same author

Heterogeneous effect of leucotriene CysLT1 receptor antagonists on antigen-induced motor and inflammatory responses in guinea-pig airways.

Autonomic & autacoid pharmacology·2007
Same author

In vitro and in vivo activity of analogues of the kinin B2 receptor antagonist MEN1 1270.

Canadian journal of physiology and pharmacology·2002

Area of Science:

  • Neuroscience
  • Urology
  • Pharmacology

Background:

  • The micturition reflex is a complex process involving both central and peripheral neural pathways.
  • Nociceptin, an endogenous ligand, and its receptor ORL(1) have been implicated in various physiological functions, but their specific role in micturition is not fully elucidated.

Purpose of the Study:

  • To investigate the role of nociceptin and ORL(1) receptors in the regulation of the micturition reflex.
  • To determine the effects of nociceptin administration via different routes (intravenous, intrathecal, intracerebroventricular, topical) on micturition and bladder function.

Main Methods:

  • Administration of nociceptin at various doses and routes in animal models.
  • Assessment of micturition reflex activity and urodynamic parameters.

Related Experiment Videos

  • Use of capsaicin pretreatment to identify the involvement of capsaicin-sensitive nerves.
  • Co-administration of naloxone and tachykinin receptor antagonists to explore receptor involvement.

    • Main Results:

    • Intravenous (i.v.) and intrathecal nociceptin inhibited the micturition reflex in a naloxone-resistant manner, mediated by capsaicin-sensitive afferent nerves.
    • Intracerebroventricular (i.c.v.) nociceptin also inhibited the micturition reflex, suggesting supraspinal effects.
    • Topical application of nociceptin to the bladder induced dose-dependent excitatory effects, including triggering the micturition reflex and causing local bladder contractions.
    • Topical nociceptin-induced contractions were abolished by tachykinin NK(1) and NK(2) receptor antagonists.

    Conclusions:

    • ORL(1) receptors are present at multiple sites involved in micturition reflex integration.
    • Nociceptin can exert both inhibitory (central and afferent) and excitatory (peripheral) effects on the micturition reflex.
    • The route of administration and experimental conditions determine the net effect of nociceptin on bladder function.