Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Cellular calcium imaging: so, what's new?

¹Marine Biological Association of the UK, The Laboratory, Citadel Hill, Plymouth, UK PL1 2PB. cbr@mba.ac.uk

Trends in Cell Biology

|September 22, 2000

Summary

Recent advances in calcium reporting methods and instrumentation have significantly improved the imaging of intracellular calcium (Ca2+) signals. Cell biologists now have diverse tools, including fluorescent probes, to decipher complex spatiotemporal Ca2+ dynamics.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

OSPRay - A CPU Ray Tracing Framework for Scientific Visualization.

IEEE transactions on visualization and computer graphics·2016

Same author

Phytochrome and potassium uptake by mung bean hypocotyl sections.

Planta·2014

Same author

Measurement of internal pH in the coccolithophoreEmiliania huxleyi using 2',7'-bis-(2-carboxyethyl)-5(and-6)carboxyfluorescein acetoxymethylester and digital imaging microscopy.

Planta·2013

Same author

Changing views of the interconnections between the oceans and human health in Europe.

Microbial ecology·2013

Same author

Regulation of channel activity underlying cell volume and polarity signals in Fucus.

Journal of experimental botany·2011

Same author

Physically-Based Interactive Flow Visualization Based on Schlieren and Interferometry Experimental Techniques.

IEEE transactions on visualization and computer graphics·2010

Area of Science:

Cell Biology
Biophysics
Biochemistry

Background:

Intracellular calcium (Ca2+) signaling is crucial for numerous cellular processes.
Understanding the spatiotemporal dynamics of Ca2+ signals is essential for cell biology.
Previous limitations in Ca2+ detection methods hindered detailed analysis.

Purpose of the Study:

To review recent advancements in intracellular Ca2+ signal detection.
To highlight the available methods for imaging Ca2+ in living cells.
To discuss the integration of molecular biology and biophysics in Ca2+ signal research.

Main Methods:

Utilizing photoproteins for Ca2+ reporting.
Employing commercially available fluorescent Ca2+ indicators.
Developing and using novel fluorescent protein-based Ca2+ probes.
Leveraging improved measurement instrumentation for Ca2+ imaging.

Main Results:

Significant improvements in the resolution and sensitivity of Ca2+ signal detection.
A broader range of options available to cell biologists for Ca2+ imaging.
Enhanced ability to discern the complex spatiotemporal patterns of intracellular Ca2+.

Conclusions:

The combination of advanced Ca2+ reporting tools and instrumentation has revolutionized the study of intracellular Ca2+ signals.
Molecular biology and biophysics are key drivers in advancing the field of Ca2+ signal deciphering.
Future research will benefit from these integrated approaches to understand cellular functions.

Related Experiment Videos