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Updated: Oct 13, 2026

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A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
Published on: March 22, 2012
Real-time imaging of two-photon-induced fluorescence with a microlens-array scanner and a regenerative amplifier
K Fujita1, O Nakamura, T Kaneko
1Department of Applied Physics, Osaka University, Suita, Japan. fujita@ap.eng.osaka-u.ac.jp
Journal of Microscopy
|September 22, 2000
Abstract:
A real-time two-photon fluorescence microscope with a microlens-array scanner using a regenerative amplifier is presented. The regenerative amplifier generates pulsed laser lights with extremely high peak power and produces brighter two-photon-induced fluorescence compared with that produced without the amplifier. Experimental results obtained from the observation of some biological samples with the proposed microscope are given.
