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Systemic silencing signal(s).

M Fagard1, H Vaucheret

  • 1Laboratoire de Biologie Cellulaire, INRA, Versailles, France.

Plant Molecular Biology
|September 22, 2000
PubMed
Summary
This summary is machine-generated.

Transgenic plants generate a sequence-specific signal that moves through the plant, silencing homologous genes. This systemic gene silencing, similar to natural virus resistance, protects against secondary infections.

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Area of Science:

  • Plant molecular biology
  • Genetics
  • Biochemistry

Background:

  • Transgenic plants can exhibit gene silencing phenomena like co-suppression and post-transcriptional gene silencing (PTGS).
  • Viral infection in plants can trigger a phenomenon known as virus-induced gene silencing (VIGS).
  • Both PTGS and VIGS involve sequence-specific RNA degradation and can lead to systemic effects.

Purpose of the Study:

  • To investigate the nature of the systemic silencing signal produced in transgenic plants undergoing PTGS.
  • To compare the mechanisms of systemic PTGS and VIGS with natural recovery from virus infection.
  • To explore the potential role of mobile molecules in transgene-induced gene silencing.

Main Methods:

  • Grafting experiments were used to demonstrate the systemic nature of the silencing signal.

Related Experiment Videos

  • Transgenic plants expressing homologous transgenes were subjected to co-suppression or PTGS.
  • Viral infection assays were performed on transgenic plants carrying transgene sequences.
  • Comparisons were made between silencing in transgenic plants and recovery responses in non-transgenic plants.

    • Main Results:

    • Transgenic plants undergoing co-suppression or PTGS produce a sequence-specific, mobile silencing signal that moves cell-to-cell and long distances.
    • Virus infection carrying transgene sequences induces global silencing (VIGS) of integrated transgenes, despite localized viral infection.
    • Systemic PTGS and VIGS share similarities with natural recovery from virus infection, conferring protection against secondary infections.

    Conclusions:

    • A sequence-specific mobile signal, likely RNA-based, mediates systemic gene silencing in transgenic plants.
    • Systemic PTGS and VIGS represent plant defense mechanisms that can protect against viral pathogens and transgene expression.
    • The findings suggest that RNA-dependent RNA polymerase may play a role in generating these mobile silencing signals.