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FT-IR microspectroscopy for microbiological studies.

F Orsini1, D Ami, A M Villa

  • 1UdR INFM di Milano Bicocca e Dipartimento di Biotecnologie e Bioscienze, Università di Milano Bicocca, Piazza della Scienza 2, 20126, Milan, Italy.

Journal of Microbiological Methods
|September 23, 2000
PubMed
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Fourier transform infrared (FT-IR) microspectroscopy rapidly analyzes microbial microcolonies. This method reveals differences in dextrose uptake between central and peripheral cells in Candida albicans microcolonies.

Area of Science:

  • Microbiology
  • Spectroscopy
  • Biophysics

Background:

  • Candida albicans microcolonies serve as a model for microbial studies.
  • Understanding cellular metabolic activity within microcolonies is crucial.
  • Assessing nutrient uptake in microbial communities presents challenges.

Purpose of the Study:

  • To investigate Candida albicans microcolonies using infrared microspectroscopy.
  • To analyze spectral differences within microcolonies and relate them to metabolic activity.
  • To present FT-IR microspectroscopy as a rapid method for evaluating nutrient uptake.

Main Methods:

  • Fourier transform infrared (FT-IR) absorption microspectroscopy was employed.
  • Spectra were collected from microcolonies on zinc selenide (ZnSe) windows.

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  • Attenuated total reflection (ATR) microscopy was also explored for highly absorbing samples.

    • Main Results:

    • Distinct FT-IR spectra were obtained from central and peripheral regions of microcolonies.
    • Spectral variations correlated with differences in dextrose uptake.
    • Dextrose uptake was lower in central cells compared to peripheral cells.

    Conclusions:

    • FT-IR microspectroscopy provides a rapid and effective method for analyzing microbial microcolonies.
    • The technique can differentiate metabolic states within microcolonies based on nutrient uptake.
    • ATR microscopy offers a novel approach for studying dense microbial structures.