Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Enantiomer separation by capillary electrochromatography on a cyclodextrin-modified monolith.

D Wistuba1, V Schurig

  • 1Institute of Organic Chemistry, University of Tübingen, Germany. dorothee.wistuba@uni-tuebingen.de

Electrophoresis
|September 23, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sex-specific activity of (R)-(-)- and (S)- (+)-1,7-dioxaspiro[5.5]undecane, the major pheromone ofDacus oleae.

Journal of chemical ecology·2013
Same author

Male pheromone of swift moth,Hepialus hecta L. (Lepidoptera: Hepialidae).

Journal of chemical ecology·2013
Same author

Use of derivatized cyclodextrins as chiral selectors for the separation of enantiomers by gas chromatography.

Annales pharmaceutiques francaises·2010
Same author

A mixed stationary phase containing two versatile cyclodextrin-based selectors for the simultaneous gas chromatographic enantioseparation of racemic alkanes and racemic alpha-amino acid derivatives.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2008
Same author

The control of the nitrogen inversion in alkyl-substituted diaziridines.

Chemistry (Weinheim an der Bergstrasse, Germany)·2004
Same author

Accumulation of S(+) enantiomer in human beings after general anaesthesia with isoflurane racemate.

European journal of anaesthesiology·2002

A novel chiral monolithic stationary phase was developed for enantiomer separation. Monolithic capillary electrochromatography (CEC) demonstrated significantly higher efficiency compared to capillary liquid chromatography (LC).

Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Chiral separations are crucial in pharmaceuticals and chemical industries.
  • Development of stable and efficient chiral stationary phases is an ongoing challenge.

Purpose of the Study:

  • To prepare a highly stable chiral monolithic stationary phase.
  • To evaluate its performance in enantiomer separation using monolithic capillary electrochromatography (rod-CEC).
  • To compare rod-CEC with monolithic capillary liquid chromatography (rod-LC).

Main Methods:

  • Preparation of a porous silica monolith.
  • Polymer-coating with Chirasil-Dex (permethylated beta-cyclodextrin derivative).
  • Thermal immobilization of the chiral phase.
  • Enantiomer separation using rod-CEC and rod-LC.

Related Experiment Videos

Main Results:

  • A highly stable chiral monolith (up to 30 kV, >400 bar) was successfully prepared.
  • Feasible enantiomer separation of various chiral compounds was achieved by rod-CEC.
  • Rod-CEC exhibited 2-3 times higher efficiency than rod-LC.
  • Pressure-driven flow and organic modifier composition significantly impacted separation performance.

Conclusions:

  • The developed chiral monolithic stationary phase offers high stability and efficiency for enantiomer separations.
  • Rod-CEC is a superior technique to rod-LC for chiral separations using this monolithic phase.
  • Optimization of mobile phase composition is key for maximizing efficiency and resolution.