A novel electrophoretic fractionation of Escherichia coli envelopes

Particulate fractions of Escherichia coli have been submitted to electrophoretic fractionation in a buffer stabilized by sucrose gradient. Inner membrane and outer membrane were readily resolved. A combination of electrophoresis, fractional centrifugation and gel filtration can remove remaining contamination by ribosomes and cytoplasm. The presence of particles containing no phospholipids was detected after differential centrifugation. The nature of this fraction is unknown. The inner membrane exhibited heterogeneity on electrophoresis.