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Monoclonal antibodies generated against recombinant ATM support kinase activity
K J Alligood1, M Milla, N Rhodes
1Department of Cancer Biology, Glaxo Welcome Research and Development, Research Triangle Park, NC 27709-3398,USA.
Hybridoma
|September 23, 2000
Abstract:
We report on the rapid generation of two monoclonal antibodies, ATM A16.35 and ATM D16.11, that bind to the kinase domain of mutated ataxia telangiectasia (ATM). These antibodies were generated against E. coli-expressed recombinant protein using the RIMMS strategy. We show that ATM A16.35 binds ATM by Western blot analysis, and ATM D16.11 forms immune complexes with native ATM in immunoprecipitations without neutralizing kinase activity.