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Related Experiment Videos

High-throughput quantitative bioanalysis by LC/MS/MS.

M Jemal1

  • 1Bioanalytical Research, Metabolism and Pharmacokinetics, Bristol-Myers Squibb Pharmaceutical Research Institute, New Brunswick, NJ 08903-0191, USA. jemalm@bms.com

Biomedical Chromatography : BMC
|September 26, 2000
PubMed
Summary
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This review covers recent high-throughput bioanalysis advancements using liquid chromatography-tandem mass spectrometry (LC/MS/MS). Key improvements in sample preparation and mass spectrometry enhance drug and biomolecule quantitation in biological samples.

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Pharmacology

Background:

  • High-throughput bioanalysis is crucial for quantitating drugs, metabolites, and biomolecules in biological matrices.
  • Traditional methods face challenges in speed and efficiency for large sample volumes.

Purpose of the Study:

  • To review recent significant advances in sample preparation and mass spectrometry for high-throughput bioanalysis.
  • To discuss techniques enhancing speed, efficiency, and sensitivity in LC/MS/MS quantitative bioanalysis.

Main Methods:

  • Automated 96-well extraction (SPE, LLE, protein precipitation).
  • Fast-flow on-line extraction techniques.
  • Advancements in LC/MS/MS instrumentation and chromatographic separation.

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Main Results:

  • Automated extraction and fast-flow on-line methods significantly increase sample throughput.
  • Pierceable caps improve analysis speed and sample handling safety.
  • Understanding of atmospheric pressure ionization mass spectrometry is evolving, with some counter-intuitive findings.

Conclusions:

  • Recent innovations in sample preparation and LC/MS/MS are revolutionizing quantitative bioanalysis.
  • Further improvements in speed and sensitivity are expected from multiplexed systems and capillary LC.