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[Reconstruction of humoral response to sperm antigens in scid mice]
B Grygielska1, D Fiszer, A Domagała
1Zakładu Genetyki Człowieka PAN w Poznaniu.
Objective And Design:
Production of specific human antisperm antibodies by using human-SCID mice model with deposited peripheral blood lymphocytes.
Materials And Methods:
Human peripheral blood lymphocytes (PBL's; CD8(+)-negative cell fraction) were grafted to the peritoneal cavity of severely-combined immunodeficient (SCID) mice at concentration of 20-35 x 10(6) cells per mouse. Lymphocytes were obtained from non-sensitized individual (to sperm antigen) and from in vivo primed males (vasectomized). Two sets of experiments were carried out, with 'native' (glycosylated) and enzymatically deglycosylated sperm antigenic extracts. In all applied variants, sperm antigens were administered with Complete and then with Incomplete Freund adjuvant to improve an immune response.
Results And Conclusion:
This approach allowed us to obtain better pronounced humoral antisperm response, specific to sperm deglycosylated antigens when PBL's were obtained from individuals in vivo sensitized to sperm (after vasectomy).