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Molecular cloning and expression of rat betacellulin cDNA
1Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, Tsushima-naka, Okayama, Japan. tadahrk@biotech.okayama-u.ac.jp
Biochimica Et Biophysica Acta
|September 27, 2000
Abstract:
The cDNA encoding an entire open reading frame of rat betacellulin has been cloned from rat kidney. Expression of this cDNA in COS7 cells showed a significant amount of mitogenic activity in the culture media. Western blotting of the cell lysates suggested that the membrane-anchored precursor was cleaved to release its ectodomain very efficiently.
