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Updated: Aug 18, 2026

Mating and Tetrad Separation of Chlamydomonas reinhardtii for Genetic Analysis
Published on: August 13, 2009
Nucleotide sequence, genomic organization and cell-cycle-dependent expression of a Chlamydomonas 14-3-3 gene
J Voigt1, I Liebich, J Wöstemeyer
1Physiologisch-chemisches Institut, Universität Tübingen, Hoppe-Seyler-Strasse, Tübingen, Germany. juergen.voigt@uni-tuebingen.de
Abstract:
Members of the 14-3-3 protein family have been identified as regulatory elements in intracellular signalling pathways and cell cycle control. Previously we reported the nucleotide sequence of a 14-3-3 cDNA cloned from the unicellular green alga Chlamydomonas reinhardtii. In this communication, we describe the nucleotide sequence, the genomic organization and the cell-cycle-dependent expression of the corresponding gene. The coding sequence of this gene was found to be interrupted by four introns of 124, 116, 81, and 659 bp, respectively. Introns 2-4 were found in conserved positions as compared to the Arabidopsis 14-3-3 genes. A counterpart to intron 1 absent in the Arabidopsis 14-3-3 genes was found in the human 14-3-3 epsilon gene.
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