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[Detection of tubercle bacilli in clinical samples using genetic methods(PCR)compared with Lowenstein-Jensen culture
M Boruń1, M Rybczyńska, I Pawłowska
1Centrum Mikrobiologii i Wirusologii, Polskiej Akademii Nauk w Lodzi.
Pneumonologia I Alergologia Polska
|September 27, 2000
Abstract:
The aim of this work was to compare data obtained using PCR assay for detection of Mycobacterium tuberculosis with culture. We report a test for detection of tubercle bacilli by PCR and identification at species level by capture plate hybridisation and enzyme-linked immunoassay. 222 clinical samples obtained from patients with confirmed and suspected tuberculosis were analysed. These specimens were tested parallelly by conventional culture on Löwenstein-Jensen slants and PCR test. For 205 samples a complete agreement between these methods was observed.